GENZ SARS-CoV-2 Real-Time PCR Kit detects the presence of two different and highly specific gene sequences of SARS-CoV-2: N1 and N2 regions of the Nucleocapsid gene.
GENZ SARS-CoV-2 Real-Time PCR Kit
GENZ SARS-CoV-2 Real-Time PCR Kit is an in vitro nucleic acid amplification assay for the qualitative detection of SARS-CoV-2 in nasopharyngeal/oropharyngeal swabs, broncho-alveolar lavage (BAL), using BIO-RAD CFX96, Rotor-Gene Q, and QuantStudio 5 Real-time PCR Systems.
A non-infectious positive control and internal control are included. Internal control is needed to ensure RNA extraction, reverse transcription, and all reagents involved. GENZ SARS-CoV-2 Real-Time PCR Kit master mix contains primers and probes for the endogenous human target (RNase P). It is not essential for an additional external DNA or RNA template as extraction control since the human target is obtained during extraction. The positive control is used to confirm the function of the assays and overall PCR performance; the negative human extraction control is included to evaluate the quality of RNA isolation independently from the SARS-CoV-2 assays.